Bt strain S2160-1 was found to produce crystalline toxins with high insecticidal effects against mosquitoes (Culex quinquefasciatus and Aedes albopictus), which was characterized from the Bt isolate (Isolate No: 20070720S2160) from soil samples collected from Guangxi Dawangling Natural Reserves of Guangxi Province of China in 2007. Bt strain S2160-1 was stocked in the HITAR Bacillus Collections with Accession No 20070720S2160 and owned by HITAR. Scanning electron microscope observation showed that the spherical shape crystals of Bt S2160-1 with size at one fourth volume of spore to that of reference strain of Bti. SDS-PAGE analysis showed that the intact parasporal protein of Bt S2160-1 containing four major polypetide of about 140kDa, 130kDa, 75kDa and 30kDa was different from that of Bti. Furthermore, bioassays exhibited high toxicity to mosquito larva of Culex quinquefasciatus and Aedes albopictus. There are a great of differences in terms of the large plasmid patterns, parasporal proteins profiles and cry genes contents between Bt S2160-1 and Bti. The cloned genes of cry50Ba1 and cry54Ba1 expressed in BL21 (DE3) cells, but not success in Cry 30Ea1 and Cry 30Ga. Thus, it is believed that Bt S2160-1 could be applied into mosquito control as the potential alternative to prevent mosquito from developing the resistance to the toxins of Bti.