Wenfei Zhang

College of Life Sciences, Hainan Normal University, Haikou, 570206, Hainan, China
Author    Correspondence author
Bt Research, 2024, Vol. 15, No. 4   doi: 10.5376/bt.2024.15.0020
Received: 27 Jun., 2024    Accepted: 12 Aug., 2024    Published: 28 Aug., 2024

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Zhang W.F., 2024, Metabolic engineering of Bt for enhanced production of insecticidal proteins, Bt Research, 15(4): 204-214 (doi: 10.5376/bt.2024.15.0020)

Bacillus thuringiensis (Bt) is a key bacterial strain used for producing bioinsecticides, with its insecticidal proteins being widely applied in agricultural pest control. However, traditional Bt production faces challenges such as low protein yield and high production costs. To address these issues, this study explores strategies to enhance Bt metabolic pathways through metabolic engineering, aiming to increase protein production. The research covers Bt's primary and secondary metabolic pathways, their relationship with protein synthesis, and case studies of enhanced protein production achieved through genetic modification and metabolic flux optimization. This study aims to provide feasible improvements for the commercial production and application of Bt products in agriculture by offering theoretical insights into the metabolic engineering of Bt.

Bacillus thuringiensis; Insecticidal proteins; Metabolic engineering; Genetic modification; Agricultural application