Yinghua Chen , Zhongqi Wu

Institute of Life Sciences, Zhejiang A&F University, Zhuji, 311800, Zhejiang, China
Author    Correspondence author
Bt Research, 2024, Vol. 15, No. 3   
Received: 10 Mar., 2024    Accepted: 28 Apr., 2024    Published: 15 May, 2024

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

The study investigates the genetic diversity and plasmid profiles of Bacillus thuringiensis (Bt) isolates collected from various ecological niches. Bt is a widely used biopesticide due to its insecticidal properties, which are largely attributed to the presence of plasmid-borne genes encoding Cry and Vip proteins. This research aims to compare the plasmid profiles of Bt isolates from different habitats to understand their genetic diversity and potential for biocontrol applications. The study utilized techniques such as whole genome sequencing, PCR amplification, and bioassays to characterize the isolates. Results revealed significant genetic diversity among the isolates, with multiple unique plasmid profiles identified. The findings suggest that different habitats harbor distinct Bt strains with varying plasmid compositions, which could be exploited for developing novel biopesticides. This comparative analysis enhances our understanding of Bt genetic diversity and its implications for sustainable pest management.

Bacillus thuringiensis; Plasmid profiles; Genetic diversity; Biopesticides; Insecticidal proteinse