Xiuhua Liu , Jie Zhang

Biotechnology Research Center of Cuixi Academy of Biotechology, Zhuji, 311800, Zhejiang, China
Author    Correspondence author
Bt Research, 2024, Vol. 15, No. 2   doi: 10.5376/bt.2024.15.0006
Received: 08 Jan., 2024    Accepted: 20 Feb., 2024    Published: 15 Mar., 2024

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Liu X.H., and Zhang J., 2024, CRISPR-Cas9 technology in Bt genome editing and functional studies, Bt Research, 15(2): 53-64 (doi: 10.5376/bt.2024.15.0006)

CRISPR-Cas9 technology, with its unparalleled precision and efficiency, has revolutionized the field of genome editing. In Bacillus thuringiensis (Bt), widely used as a biopesticide, genome editing holds great promise for significantly enhancing its efficacy and functional understanding. This study provides an overview of the mechanisms and advancements of CRISPR-Cas9, comparing it with other genome editing technologies. It delves into its applications in Bt, including gene knockout, knock-in strategies, multiplex genome editing, and targeted mutagenesis. Additionally, it explores the functional studies of Bt genes, covering gene function identification, overexpression, gene silencing, and toxin gene analysis. This study discusses methodological advancements and challenges such as delivery methods, off-target effects, and optimization. Case studies showcase successful gene editing examples, insights gained, and best practices. It also examines ethical and regulatory issues as well as public perception. Finally, it discusses future directions, emerging trends, novel applications, and the potential impact on industrial and agricultural biotechnology. Continuous research and development in CRISPR-Cas9 technology are crucial to fully realizing its potential in Bt genome editing.

CRISPR-Cas9; Bacillus thuringiensis; Genome editing; Gene knockout; Biopesticides