Identification and Characterization of  Bacillus thuringiensis  Strain BRC-ZLL13 with High Toxic Activity Against Mosquitoes

Liu Zhaoxia<sup>1</sup>; Li Mingwei<sup>1</sup>; Wu Songqing<sup>1,2</sup>; Ye Wenhui<sup>1</sup>; Yu Xiongze<sup>1</sup>; Lin Qiuqiu<sup>1</sup>; Guan Xiong<sup>1</sup>; Zhang Lingling<sup>1</sup>

Research Report

Identification and Characterization of Bacillus thuringiensis Strain BRC-ZLL13 with High Toxic Activity Against Mosquitoes

Liu Zhaoxia¹

, Li Mingwei¹

, Wu Songqing^1,2

, Ye Wenhui¹

, Yu Xiongze¹

, Lin Qiuqiu¹

, Guan Xiong¹

, Zhang Lingling¹

1 Key Laboratoryof Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, 350002
2 Collegeof Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002

Author

Correspondence author
Bt Research, 2014, Vol. 5, No. 5 doi: 10.5376/bt.2014.05.0005
Received: 01 Sep., 2014 Accepted: 01 Oct., 2014 Published: 01 Nov., 2014

This article was first published in Genomics and Applied Biology in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Preferred citation for this article:

Liu Z.X., Li M.W., Wu S.Q., Ye W.H., Yu X.Z., Lin Q.Q., Guan X., and Zhang L.L., 2014, Identification and Characterization of Bacillus thuringiensis Strain BRC-ZLL13 with High Toxic Activity Against Mosquitoes, Bt Research, 5(5): 1-8 (doi: 10.5376/bt.2014.05.0005)

Abstract

Bacillus thuringiensis strain BRC-ZLL13 was isolated from leaves of Magnolia aenudate. It was characterized based on its insecticidal activity against Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus larvae, flagellar antigen serology, biochemical reactions, antibiotic sensitivity, plasmid profile and crystal protein profile as well as PCR analysis using novel general and specific primers for cry and cyt genes analysis. It showed more than twofold higher mosquitocidal activity against A. aegypti, A. albopictus and C. quinquefasciatus larvae than B. thuringiensis subsp. israelensis (IPS82) and displayed high similarities with the IPS82 with regard to protein and the presence of mosquitocidal genes, but had different plasmid profiles and higher growth rate. These results suggested that the loss of plasmid might be contribute to high growth rate of this strain, and indicated a potential for developing this strain for application in the fields of public hygiene and biological control.