Shancun Bao¹ , wulatai Xia² , Guangjing Yin³ , Xiaozhu A⁴ , Luchao Bai¹

1 College of Agricultural and Animal Husbandry, Qinghai University, Xining, 810016, China
2 Tongren County Forestry Workstation, Huangnan, 811300, China
3 Qinghai Forest Seedling Station, Xining, 810000, China
4 Prefecture Forestry Bureau Jiangxi Forest Farm in Yushu, Qinghai, Yushu, 815000, China
Author    Correspondence author
Molecular Pathogens, 2022, Vol. 13, No. 2   doi: 10.5376/mp.2022.13.0002
Received: 31 Mar., 2022    Accepted: 10 Apr., 2022    Published: 29 Apr., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Bao S.C., Xiawulatai, Yin G.J., A X.Z., and Bai L.C., 2022, Isolation and identification of pathogens of poplar stem diseases, Molecular Pathogens, 13(2): 1-6 (doi: 10.5376/mp.2022.13.0002)

Since 2017, Populus cathayana in Shuangshu Nursery, Huzhu County, Qinhai Province has suffered from extensive necrosis of branches, yellow pith tissue, cracks from bark to pith in severe cases, brown mucus outflow in summer, yellowish brown pith, and no obvious disease. In this study, symptom observation, specimen collection, pathogen isolation and culture, morphological observation and molecular biological identification were carried out on poplar branch diseases. After the pathogen was isolated, cultured and purified, a pure bacterial strain H1 was obtained and its pathogenicity was tested. The results showed that the pathogenic bacterium H1 caused the disease of Populus cathayana plants, which was consistent with the disease symptoms in the field. Through morphological observation and molecular sequence analysis, the pathogen H1 was identified as Pantoea agglomerans, which can cause poplar bacterial canker. This study identified the pathogen of Populus cathayana branch disease in Huzhu country, which can provide basis for the prevention and control of the disease.

Populus cathayana; Pathogen identification; Pantoea agglomerans