Construction of F1 Populations of S. spontaneum L. and Sugarcane and Molecular Detection of Genetic Diversity
Ministry of Agriculture of Guangdong Sugarcane Germplasm Resources and Utilization of Scientific Observation Test Station, Hainan Sugarcane Breeding Station of GSIRI, Sanya, 572025, China
Molecular Pathogens, 2021, Vol. 12, No. 1 doi: 10.5376/mp.2021.12.0001
Received: 10 Nov., 2021 Accepted: 16 Nov., 2021 Published: 25 Nov., 2021
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This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Guo Y.Q., Fu C., Xu H.Y., Chang H.L., Wu J.T., Qiu Y.S., and Wang Q.N., 2021, Construction of F1 Populations of S. spontaneum L. and sugarcane and molecular detection of genetic diversity, Molecular Pathogens, 12(1): 1-7 (doi: 10.5376/mp.2021.12.0001)
In order to obtain more hybrid offspring with excellent traits of Saccharum spontaneum L., this research used the YN 82-116 as a female parent to hybridize with the male parent GT 05-3595. The SSR primer MSSCIR1 combined with capillary electrophoresis technology was used to identify true and false hybrids of 48 hybrid progenies and to analyze genetic diversity. A total of 28 SSR fragments were amplified, the polymorphic bands ratio was 67.9%, and the F1 generations were all real hybrids. The parental and offspring inheritance similarity coefficients range from 0.00 to 0.97, with an average of 0.58. The overall genetic similarity of filial generation is high, but the genetic differences are large. UPGMA cluster analysis showed that 37 offspring were clustered with their father, with a closer genetic distance and higher genetic similarity. The results can be used in the later stage to guide the process of cross breeding, improve breeding efficiency, and effectively introduce germplasm resources that are densely S. spontaneum L.
GT05-3595; Saccharum spontaneum L.; Capillary electrophoresis; Genetic diversity