A. Lakshmi² , S. Ramesh² , K. Chairman¹ , M. Jeyamala¹ , S. Sankar³ , A. Murugan¹

1. Department of Advanced Zoology and Biotechnology, Sri Paramakalyani College, Alwarkurichi, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu, India - 627 412
2. Department Microbiology, Sri Paramakalyani College, Alwarkurichi, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu, India - 627 412
3. CAS in Marine Biology, Annamalai University, Parangipettai – 608502
Author    Correspondence author
Molecular Microbiology Research, 2013, Vol. 3, No. 2   doi: 10.5376/mmr.2013.03.0002
Received: 19 Mar., 2013    Accepted: 27 Mar., 2013    Published: 26 Apr., 2013

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Ramesh et al., 2013, Carcinogenic Assay of Foods Using Auxotrophic Strains of Salmonella typhimurium Employing Reverse Mutation Technique, Molecular Microbiology Research, Vol.3, No.2 9-20 (doi: 10.5376/mmr.2013.03.0002)

World hastens the changes in food items, that facilities of the addition of colouring agents and flavouring agents. They were not aware of the treat of dangers awaiting them in the form of food increasing with modernization in food along with the development new diseases many desirable factors are involved in foods. Fifty five food samples were tested by the reverse Mutation test for the presence of carcinogenic chemicals. The auxotrophic mutant strain had the character of his arg+ , amp+ and Tet +. From the result of reverse mutation assay, we informed that 48% of sample was under 2+ level, 40% of sample under 1+ level were recorded for carcinogenic behavior while 59.2% the sample did not reflect any level. Out of 55 samples, 41.8% showed the positive result that indicated dangerous carcinogenic chemicals in fried oil, reheated oil, halwa, jangiri etc., Even sweets had the high content of carcinogens which were mostly preferred by kids. The extra chromosomal DNAs isolated were electrophoresed.

Uv-mutagenic; Food samples; Tetracycline; Arginine; Amest test