Diversity of Endophytic Fungi Isolated from Gentiana rhodantha and Screening for Biocontrol Stains
School of Biological Science and Technology, Liupanshui Normal University, Liupanshui 553004, China
Molecular Microbiology Research, 2021, Vol. 11, No. 1
Received: 13 Aug., 2021 Accepted: 31 Aug., 2021 Published: 10 Sep., 2021
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This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
In order to clarify the composition of endophytic fungi in Gentiana rhodantha in colonization tissues with four Liupanshui sampling sites (Fenghuang Zhongshan, Nankai Shuicheng, Yangmei Shuicheng and Xinchang Liuzhi) in Liupanshui, strains were isolated with health leaf, stem and root and identification based on morphological characteristics and ITS sequence analysis. As well as screening for biocontrol stains by the dural culture method on PDA plates with Sclerotinia sclerotionrum. The results showed that G. rhodantha concealed rich fungal diversity, the colonization rate and isolation rate of endophytic fungi reached 49.38% and 61.25% respectively. 294 strains of endophytic fungi were obtained from 480 explant tissue segments of G. rhodantha and belonging to 2 phylum, 5 classes, 14 orders and 32 genera; of which Fusarium and Colletotrichum were comparatively advantageous taxa in endophytic fungi of G. rhodantha, and the species contained occupied 9.86% and 8.84% of the total endophytes respectively．According to the calculation results, Xinchang LZ in four sites and stem in three tissues showed optimum degrees of diversity, evenness and dominance respectively. 23 strains of endophytic fungi with inhibitory activities against S. sclerotionrum by the dural culture method; Amomg them, the inhibition rate of Botryosphaeria sp. R022, R021, Phomopsis sp. S002, Colletotrichum sp. S028 and Fusarium sp. L031 were higher than 70% and showed the best inhibitory potential.
Gentiana rhodantha; Endophytic fungi; Diversity; Sclerotinia sclerotionrum; Biocontrol fungi
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Molecular Microbiology Research
• Volume 11