Xiumei Tan¹ , Xiang Ding² , Rongmei Yuan¹ , Yiling Hou¹

1 Key Laboratory of Southwest China Wildlife Resources Conservation, the Ministry of Education, College of Life Science, China West Normal University, Nan Chong, 637009, China
2 College of Environmental Science and Engineering, China West Normal University, Nan Chong, 637009, China
Author    Correspondence author
Molecular Microbiology Research, 2020, Vol. 10, No. 2   
Received: 12 May, 2020    Accepted: 12 May, 2020    Published: 12 May, 2020

This article was first published in Genomics and Applied Biology in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

The mgr2 gene encodes an important transmembrane protein in mitochondria, which plays an important role in cell growth and survival. The model of Schizosaccharomyces pombe and the live cell imaging was used to explore the effect of mgr2 gene deletion on cell dynamics in mitosis. The results showed that the deletion of mgr2 gene would cause the number and length of microtubules abnormality and affect microtubule dynamics at interphase. At the same time, spindle microtubule dynamics were different in mgr2Δ cells compared with wild type cells. Spindle length statistics showed that there was delayed spindle breakage in mgr2Δ cells. The mgr2 gene deletion can also affect the spindle growth rate in the metaphase，metaphase time and anaphase time. Live-cell imaging were performed on mutant strains to observe two distinct chromosome behaviors: normal and lagging. In addition, there was abnormal spindle breakage. It is concluded that the loss of mgr2 gene from mitochondria caused abnormal cell mitosis, which would contribute to the spindle maintenance deficiency, chromosome segregation deficiency, spindle breakage deficiency.

mgr2 gene; Mitosis; Cell cycle; Cell dynamics; Microtubule

(The advance publishing of the abstract of this manuscript does not mean final published, the end result whether or not published will depend on the comments of peer reviewers and decision of our editorial board.)