Zhongqi Wu^1,2

1 Institute of Life Science, Jiyang College of Zhejiang A&F University, Zhuji, 311800, Zhejiang, China
2 Cuixi Academy of Biotechnology, Zhuji, 311800, Zhejiang, China
Author    Correspondence author
Molecular Microbiology Research, 2014, Vol. 4, No. 1   doi: 10.5376/mmr.2014.04.0001
Received: 09 Oct., 2014    Accepted: 12 Nov., 2014    Published: 23 Dec., 2014

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Wu Z.Q., 2014, Analysis of Bt S3076-1 toxin protein at genome-wide level, Molecular Microbiology Research, 4(1): 1-10 (doi: 10.5376/mmr.2014.04.0001)

In order to systematically analyze the quantity, species and distribution of Bt toxin protein carried by Bt S3076-1 strain, gene prediction, local database blast and protein structure analysis of Bt S3076-1 strain were carried out at the genome-wide level in this research. A total of 45 candidate Bt toxin protein genes were identified in the genome of Bt S3076-1 strain. To further confirm the reliability of these Bt toxin protein genes, we analyzed the primary, secondary and tertiary structures of these candidate Bt toxin proteins. The results of physicochemical properties analysis showed that the length of these candidate Bt toxin proteins sequence ranged from 50 to 1 247 aa, the molecular weight of the proteins ranged from 6.02 to 141.55 kD, and the isoelectric point of the proteins ranged from 4.3 to 10.88. The pH of 21 proteins of these candidate Bt toxin proteins were greater than 7. The results of protein tertiary domain analysis and functional domain analysis of PFAM indicated that there were seven Cry toxin proteins containing typical 3-Domain endotoxin domains (Endotoxin_N, Endotoxin_M and delta_endotoxin_C) in Bt S3076-1 genome, four Cry toxin proteins containing atypical conserved domains (RICIN or Toxin_10, etc.), and one Vip toxin protein. Therefore, it was predicted that eleven genes encoding Cry toxin protein and one gene encoding Vip toxin protein might exist in Bt S3076-1 strain.

Bacillus thuringiensis; Bt S3076-1; Bt toxin protein; Cry toxin protein; Vip toxin protein