Extended-spectrum β-lactamase production in Klebsiella pneumoniae and Escherichia coli at Jimma University Specialized Hospital, South-West, Ethiopia  

Shewki Moga Siraj1 , Solomon Ali2 , Beyene Wondafrash3
1. Ethiopian Public Health Institute (EPHI), National Tuberculosis Reference Laboratory, Addis Ababa, Ethiopia
2. Department of Medical Laboratory Science and Pathology, Jimma University, Ethiopia
3. Post Graduate Study Coordinator, Jimma University, Ethiopia
Author    Correspondence author
Molecular Microbiology Research, 2015, Vol. 5, No. 1   doi: 10.5376/mmr.2015.05.0001
Received: 02 Nov., 2014    Accepted: 18 Dec., 2014    Published: 23 Jan., 2015
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Siraj et al., 2015, Extended-spectrum β-lactamase production in Klebsiella pneumoniae and Escherichia coli at Jimma University Specialized Hospital, South-West, Ethiopia, Molecular Microbiology Research, Vol.5, No.1 1-9 (doi: 10.5376/mmr.2015.04.0001)

Abstract

Background: Extended spectrum β-lactamases (ESBLs) have emerged as a major threat worldwide with limited treatment options. The prevalence of ESBL producing Escherichia coli and Klebsiella pneumoniae strains largely remain unknown inEthiopia.
 

Objectives: The study was aimed to determine the occurrence of extended spectrum β-lactamase-producing E. coli and K. pneumoniae among inpatient and outpatient settings, their antimicrobial resistance profile and associated risk factors in Jimma University Specialized Hospital (JUSH).
 

Methods: Laboratory based comparative cross-sectional study design was conducted from September 2011 to February 2012.
 

Result: A total of 471 consecutive, non repetitive clinical specimens were collected among inpatients (n=314) and outpatients (n=157). Among these, 112 isolates of K. pneumoniae (n=27) and E. coli (n=85) were recovered. Overall prevalence of extended spectrum beta lactamase (ESBL) producers was 38.4% (n=43) of total isolates. Extended spectrum beta lactamases were found in 28.2% (n=24) of E. coli and 70.4 % (n=19) of K. pneumoniae. Extended spectrum beta lactamase producers mediated very high resistance to both beta-lactams and non-betalactams and they were significantly higher among isolates from in-patients (46.4%) than out-patients (14.3%). On multivariare analysis, treatment with third generation cephalosporin was identified as a sole risk factor for acquisition of ESBL enzyme.
 

Conclusions: Our findings confirmed that infection due to extended spectrum beta lactamase -producing E. coli and K. pneumonia is prevalent in Jimma University Specialized Hospital (JUSH) and exposure to third generation cephalosporin was associated with these infections. Resistance of these isolates to antibiotics was also higher among inpatients.

Keywords
Escherichia coli; Klebsiella pneumoniae; Extended spectrum β-lactamases; Inpatients; Outpatients
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