The Study of Loss of Mitochondrial Membrane Protein mgr2 Gene Results in Changes of Cell Dynamics in Fission Yeast
1 Key Laboratory of Southwest China Wildlife Resources Conservation, the Ministry of Education, College of Life Science, China West Normal University, Nan Chong, 637009, China
2 College of Environmental Science and Engineering, China West Normal University, Nan Chong, 637009, China
Molecular Microbiology Research, 2020, Vol. 10, No. 2
Received: 12 May, 2020 Accepted: 12 May, 2020 Published: 12 May, 2020
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This article was first published in Genomics and Applied Biology in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The mgr2 gene encodes an important transmembrane protein in mitochondria, which plays an important role in cell growth and survival. The model of Schizosaccharomyces pombe and the live cell imaging was used to explore the effect of mgr2 gene deletion on cell dynamics in mitosis. The results showed that the deletion of mgr2 gene would cause the number and length of microtubules abnormality and affect microtubule dynamics at interphase. At the same time, spindle microtubule dynamics were different in mgr2Δ cells compared with wild type cells. Spindle length statistics showed that there was delayed spindle breakage in mgr2Δ cells. The mgr2 gene deletion can also affect the spindle growth rate in the metaphase，metaphase time and anaphase time. Live-cell imaging were performed on mutant strains to observe two distinct chromosome behaviors: normal and lagging. In addition, there was abnormal spindle breakage. It is concluded that the loss of mgr2 gene from mitochondria caused abnormal cell mitosis, which would contribute to the spindle maintenance deficiency, chromosome segregation deficiency, spindle breakage deficiency.
mgr2 gene; Mitosis; Cell cycle; Cell dynamics; Microtubule
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