Research Article

Mining New Bt Insecticidal Protein Genes Resource Using Illumina Sequencing Technology  

Zhang Wenfei1 , Wu Hongping1 , Xu Zhixia1 , Jin Yinghong1 , Wu Zhongqi1 , Qian Jiangzhao1 , Huang Nannan1 , Jin Wenjie1 , Jia Luyu1 , Li Xinfeng1 , Wang Ning1 , Zhao Cong2 , Liu Xiaojing1 , Liu Jie1 , Liu Mingyue1 , Liu Yifeng1 , Wang  Ruiping1
1 Ministry of Education Key Laboratory for Tropical Animal and Plant Ecology, College of Life Sciences, Hainan Normal University, Haikou, 571158
2 Hannan Institude of Tropical Agricultural Resource, Sanya, 572025
Author    Correspondence author
Bt Research, 2014, Vol. 5, No. 4   doi: 10.5376/bt.2014.05.0004
Received: 01 Aug., 2014    Accepted: 01 Sep., 2014    Published: 01 Oct., 2014
© 2014 BioPublisher Publishing Platform
This article was first published in Genomics and Applied Biology in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Zhang W.F., Wu H.P., Xu Z.X., Jin Y.H., Wu Z.Q., Qian J.Z., Huang N.N., Jin W.J., Jia L.Y., Li X.F., Wang N., Zhao C., Liu X.J., Liu J., Liu M.Y., Liu Y.F., and Wang R.P., 2014, Mining New Bt Insecticidal Protein Genes Resource Using Illumina Sequencing Technology, Bt Research, 5(4): 1-6 (doi: 10.5376/bt.2014.05.0004 )

Abstract

Bacillus thuringiensis (Bt), as the most widely used biologically produced pest control agent, is already auseful alternative or supplement to synthetic chemical pesticide and is also a key source of genes for transgenic expression to provide pest resistance in plants. On basis of Bt strains isolated from soil samples from the Diaoluoshan and Wuzhishan Natural Reserve, we carried out a large-scale screening the insecticidal genes from the special Btstrains through Illumina sequencing. In this study, 25 of the insecticidal genes, including 5 of the novel vegetative insecticidal protein genes (vip) and 3 of the new cyt genes had been identified from the different Bt strains. All the result indicated that the second generation sequencing technology of illumine is efficient in mining the insecticidal gene from Bt isolates, which would further enrich the new different insecticide genes application in commercial agriculture, forest management, and mosquito control.

Keywords
Illumina sequencing; Bacillus thuringiensis; Insecticide proteins
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. Zhang Wenfei
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