A Letter
Bt A Novel Strain with Larvacidal Toxicity Against Lepidopteran Insects 
2 Institute of Life Science, Jiyang College of Zhejiang A&F University, Zhuji, 311800, Zhejiang, China
3 Cuixi Academy of Biotechnology, Zhuji, 311800, Zhejiang, China
Author Correspondence author
Bt Research, 2017, Vol. 8, No. 1 doi: 10.5376/bt.2017.08.0001
Received: 25 Jan., 2017 Accepted: 26 Mar., 2017 Published: 24 May, 2017
Wu Z.Q., 2017, Bt S3076-1, A novel strain with larvacidal toxicity against Lepidopteran insects, Bt Research, 8(1): 1-2 (doi: 10.5376/bt.2017.08.0001)
Bt S3076-1 Strain, Isolate No: 20070720S3076, was isolated from the soil that sampled from the Diaoluoshan National Nature Reserve in Hainan island of China by Hainan Institute of Tropical Agricultural Resources (HITAR) in 2007. Scanning electron microscope showed that the strain can generate high-yielding spherical crystal and square crystal during the later sporulation. SDS-PAGE analysis showed that the protein profile mainly contains six protein bands with the molecular weight about 140kDa, 90 kDa, 70 kDa, 50 kDa, 45 kDa and 25 kDa. The genome-wide predictive analysis indicated that this strain might contain 12 toxic protein genes, including cry35Ac-like, cry35Ab-like, cry39Ab-like, cry39Ab-like, cry19-like, cry56Aa-like, cry1Aa3-like, cry1Ba-like, cry41Aa1-like, cry31Aa6-like, vip1Ac-like and cry54-like. The bioassays carried out on Lepidopteran cotton bollworm (Helicoverpa armigera Hubner) exhibited that the strain Bt S3076-1 has significantly toxic to third instar larva of cotton bollworm.
1 General Description
Bt Strain: S3076-1
Isolate: 20070720S1028
Isolate source: Soil
Collected By: Wenfei Zhang, Liu Xie, Fang Xuanjun
Collection Date: July-20-2007
Collection Site: Diaoluoshan National Nature Reserve in Hainan, China
Subspecies: Unknown
Serovariety: Unknown
Identified By: Zhongqi Wu, Xuanjun Fang
Deposited At: the HITAR Bacillus Collections with Accession No 20070720S3076; China General Microbiology Culture Collection Center (CGMCC), Accession No: CGMCC No.13277
Owned by: HITAR Institute of Tropical Agricultural Resources Ltd.
2 Molecular Features
Crystal shape: Spherical crystal, Square crystal
Protein profile: 140 kDa, 90 kDa, 70 kDa, 50 kDa, 45 kDa, 25 kDa
Plasmid profile: Three plasmids of 130 kb size about
Genotype: cry35Ac-like, cry35Ab-like, cry39Ab-like, cry39Ab-like, cry19-like, cry56Aa-like, cry1Aa3-like, cry1Ba-like, cry41Aa1-like, cry31Aa6-like, vip1Ac-like, cry54-like
Cloned gene: cry35Ac-like, cry35Ab-like, cry1Aa3-like, cry1Ba-like, cry41Aa1-like, vip1Ac-like, cry54-like
Target insect: Lepidopteran insect: Cotton bollworm (Helicoverpa armigera Hubner)
3 Featured Photos
Figure 1: Morphological identification of spores and crystal protein of Bt S3076-1 strain by optical microscope
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Figure 1 Morphological identification of spores and crystal protein of Bt S3076-1 strain by optical microscope Note: Carbolfuchsin staining (100×); C: crystal protein; S: spore |
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Figure 2: Morphological identification of spores and crystal protein of Bt S3076-1 strain by SEM
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Figure 2 Morphological identification of spores and crystal protein of Bt S3076-1 strain by SEM Note: Scanning electron microscopy (SEM) observation (10.0kV, 7.4 mm×5 k); C: crystal protein; S: spore |
Figure 3: SDS-PAGE analysis of crystal protein from Bt S3076-1 strain
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Figure 3 SDS-PAGE analysis of crystal protein from Bt S3076-1 strain Note: 1~6: 140 kDa, 90 kDa, 70 kDa, 50 kDa, 45 kDa and 25 kDa |
4 Related Publication
Wu et al., 2016, Draft genome sequence of Bacillus thuringiensis strain S3076-1, Bt Research, Vol.7, No.1 1-7 (doi:10.5376/bt.2016.07.0001)
5 Filed Patent or Related to This Strain
None.
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